3/15/2023 0 Comments Moby games cooking mamaOnce more, the wild-type is shown as 10 and 30 min calcium stress versus time zero (20 hours growth), and gene deletion strains are shown as the deletion strain versus the equivalent wild-type 10 and 30 min time points (the mutant values have been normalised to the basal level of expression of each gene before stress, i.e., expression ratios are being compared: wild-type 10 min versus time zero divided by a specific mutant 10 and 30 min versus time zero). Above each graph the corresponding result of the heat-map RNAseq for each gene. Standard deviations present the average of three independent biological repetitions (each with 2 technical repetitions). Gene expression was normalized using cofA (Afu5g10570). S2 Fig: The wild-type, Δ crzA, Δ zipD, and Δ crzA Δ zipD mutant strains were grown for 16 h at 37☌ and transferred to 200 mM CaCl 2 for 0, 10, and 30 mins. Goldman, Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing 1, * Patrícia Alves de Castro, Formal analysis, Investigation, # 1 Ana Cristina Colabardini, Formal analysis, Investigation, # 1 Adriana Oliveira Manfiolli, Formal analysis, Investigation, 1 Jéssica Chiaratto, Investigation, 1 Lilian Pereira Silva, Investigation, 1 Eliciane Cevolani Mattos, Formal analysis, Investigation, 1 Giuseppe Palmisano, Formal analysis, Investigation, 2 Fausto Almeida, Formal analysis, Investigation, 3 Gabriela Felix Persinoti, Data curation, 4 Laure Nicolas Annick Ries, Formal analysis, Investigation, 3 Laura Mellado, Formal analysis, Investigation, 1 Marina Campos Rocha, Formal analysis, Investigation, 5 Michael Bromley, Resources, 6 Roberto Nascimento Silva, Formal analysis, Investigation, 3 Gabriel Scalini de Souza, Investigation, 7 Flávio Vieira Loures, Formal analysis, Investigation, 7 Iran Malavazi, Formal analysis, Investigation, 5 Neil Andrew Brown, Writing – original draft, Writing – review & editing, 8, * and Gustavo H.
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